This technique was developed to image, for the first time, the ultrastructure of the postsynaptic density in which long-term potentiation was selectively induced just seconds or minutes before, but it can be applied for the study of any biological process that requires the precise relocalization of micron-wide structures for their correlated imaging with 2PM and EM.Ĭorrelated imaging DAB Dendritic spine Photobranding Photoetching Photomarking Postsynaptic density Serial-section transmission electron microscopy Synapse Time-lapse live two-photon fluorescence microscopy. Landmarks created through a photomarking method based on the 2-photon-induced precipitation of an electrodense compound are used to unequivocally localize the stimulated synapse. This technique permits the selective stimulation and live imaging of a single dendritic spine and the subsequent localization of the same spine in EM ultrathin serial sections. Here, we describe a combination of procedures designed to achieve the correlated imaging of the same individual synapse under both 2PM and EM. Electron microscopy (EM) allows the ultrastructural imaging of subcellular components but cannot detect fluorescence and lacks temporal resolution. Two-photon fluorescence microscopy (2PM) allows time-course live imaging of individual synapses but lacks ultrastructural resolution. Synapses learn and remember by persistent modifications of their internal structures and composition but, due to their small size, it is difficult to observe these changes at the ultrastructural level in real time. 9 Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan.8 School of Life Science, South China Normal University, Guangzhou, China.7 Saitama University Brain Science Institute, Saitama University, Saitama, Japan.6 Brain Science Institute, RIKEN, Wako, Saitama, Japan.5 RIKEN-MIT Neuroscience Research Center, Massachusetts Institute of Technology, Cambridge, MA, USA.4 The Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. 3 Institute for Bioengineering of Catalonia, Barcelona, Spain. ![]() A watermark is a semi-transparent image or text that. 2 The Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. 3 is a powerful and easy-to-use software that allows you to add watermarks to your images and documents. Our built-in antivirus checked this Mac download and rated it as 100 safe. ![]() The latest setup package occupies 24.9 MB on disk.
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